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Recently, studies on “soil” and “seed” have become increasingly popular, especially in epithelial cancer cells
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Media was removed and cells were washed with cAMP stimulation buffer (Hanks balanced salt solution (HBSS), 20 mM HEPES, pH 7.4) and then incubated in cAMP stimulation buffer for 30 min at 37°C and 5% CO 2
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